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Cyclin D1 Antibody [CCND1/809]

Catalog Number: orb1252470

DispatchUsually dispatched within 5-10 working days
$ 1,070.00
Catalog Numberorb1252470
CategoryAntibodies
DescriptionCyclin D1 Antibody [CCND1/809]
Species/HostMouse
ClonalityMonoclonal
Clone NumberCCND1/809
Tested applicationsFC, IF, IHC-P, WB
ReactivityHuman
IsotypeIgG2a, kappa
ImmunogenRecombinant human protein was used as the immunogen for the Cyclin D1 antibody.
Antibody TypePrimary Antibody
Concentration0.2 mg/mL
Form/AppearanceLiquid
ConjugationUnconjugated
TargetCCND1
UniProt IDP24385
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Alternative namesG1/S-specific cyclin-D1, B-cell lymphoma 1 protein
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NoteFor research use only
Application notesFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Cyclin D1 Antibody [CCND1/809]

Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with Cyclin D1 antibody (CCND1/809).

Cyclin D1 Antibody [CCND1/809]

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cyclin D1 antibody (CCND1/809).

Cyclin D1 Antibody [CCND1/809]

Flow cytometry of human HeLa cells. Black: cells alone; Green: isotype control; Red:PE-labeled Cyclin D1 antibody (CCND1/809)

Cyclin D1 Antibody [CCND1/809]

Flow cytometry of human Jurkat cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Cyclin D1 antibody (CCND1/809).

Cyclin D1 Antibody [CCND1/809]

SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (clone CCND1/809). Confirmation of Integrity and Purity of the Antibody.