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Cyclin D1 Antibody / CCND1

Catalog Number: orb750252

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb750252
CategoryAntibodies
DescriptionCyclin D1 (CCND1), one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of Cyclin D1. This antibody is useful in identifying mantle cell lymphomas (CCND1+) from CLL/SLL and follicular lymphomas (CCND1-). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express CCND1.
Species/HostMouse
ClonalityMonoclonal
Clone NumberSPM587
Tested applicationsFACS, IHC-P, WB
ReactivityHuman, Mouse
IsotypeMouse IgG2a, kappa
ImmunogenRecombinant full length human protein was used as the immunogen for this CCND1 antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis CCND1 antibody is available for research use only.
UniProt IDP24385
StorageStore the CCND1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
Cyclin D1 Antibody / CCND1

IHC: Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with CCND1 antibody.

Cyclin D1 Antibody / CCND1

Western blot testing of 1) human HepG2, 2) mouse NIH3T3 and 3) mouse C2C12 lysate with CCND1 antibody. Expected/observed molecular weight ~32-36kDa.

Cyclin D1 Antibody / CCND1

Flow Cytometry testing of Jurkat cells. Black: cells alone; Green: isotype control; Red: PE-labeled Cyclin D1 antibody.

Cyclin D1 Antibody / CCND1

Flow Cytometry testing of MCF-7 cells. Black: cells alone; Green: isotype control; Red: PE-labeled Cyclin D1 antibody.

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