You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb750252 |
---|---|
Category | Antibodies |
Description | Cyclin D1 (CCND1), one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of Cyclin D1. This antibody is useful in identifying mantle cell lymphomas (CCND1+) from CLL/SLL and follicular lymphomas (CCND1-). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express CCND1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | SPM587 |
Tested applications | FACS, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Mouse IgG2a, kappa |
Immunogen | Recombinant full length human protein was used as the immunogen for this CCND1 antibody. |
Antibody Type | Primary Antibody |
Dilution range | Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This CCND1 antibody is available for research use only. |
UniProt ID | P24385 |
Storage | Store the CCND1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC: Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with CCND1 antibody.
Western blot testing of 1) human HepG2, 2) mouse NIH3T3 and 3) mouse C2C12 lysate with CCND1 antibody. Expected/observed molecular weight ~32-36kDa.
Flow Cytometry testing of Jurkat cells. Black: cells alone; Green: isotype control; Red: PE-labeled Cyclin D1 antibody.
Flow Cytometry testing of MCF-7 cells. Black: cells alone; Green: isotype control; Red: PE-labeled Cyclin D1 antibody.
FACS, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |