Cyclin D1 Antibody / CCND1

• Catalog Number: orb749766
• Category: Antibodies
• Description: Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CCND1/809
• Tested applications: FACS, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the Cyclin D1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Cyclin D1 antibody is available for research use only.
• UniProt ID: P24385
• Storage: Store the Cyclin D1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cyclin D1 antibody (CCND1/809).

IHC: Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with Cyclin D1 antibody (CCND1/809).

Flow cytometry of human HeLa cells. Black: cells alone; Green: isotype control; Red: PE-labeled Cyclin D1 antibody (CCND1/809).

Flow cytometry of human Jurkat cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Cyclin D1 antibody (CCND1/809).

SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (clone CCND1/809). Confirmation of Integrity and Purity of the Antibody.