Cyclin D1 Antibody

SKU: orb749766

Description

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

• Tested Applications: FACS, IHC-P
• Dilution Range: Flow cytometry: 0.5-1ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: CCND1/809
• Immunogen: Recombinant human protein was used as the immunogen for the Cyclin D1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cyclin D1 antibody (CCND1/809).

IHC: Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with Cyclin D1 antibody (CCND1/809).

Flow cytometry of human HeLa cells. Black: cells alone; Green: isotype control; Red: PE-labeled Cyclin D1 antibody (CCND1/809).

Flow cytometry of human Jurkat cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Cyclin D1 antibody (CCND1/809).

SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (clone CCND1/809). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P24385