CTSD Antibody

SKU: orb1788477

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human.
• Target: CTSD
• Molecular Weight: 44552 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cathepsin D, Cathepsin D light chain, Cathepsin D heavy chain, CTSD, CPSD

Immunohistochemical analysis of paraffin-embedded H.liver section using CTSD. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from HepG2, MCF-7, A431 cell line (from left to right), using CTSD Antibody. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded H.breast carcinoma section using CTSD. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of HepG2 cells stained with CTSD. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Quick Database Links

Gene Symbol

CTSD

UniProt

P07339