CTSD Antibody

• Catalog Number: orb1927082
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P07339
• MW: 44552 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:25, WB: 1:1000, IHC-P-Leica: 1:1000, IHC-P-Leica: 1:1000
• Antibody Type: Primary Antibody
• Clone Number: RB44366
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cathepsin D, Cathepsin D light chain, Cathepsin D
• Note: For research use only

All lanes: Anti-CTSD Antibody at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: PANC-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human liver tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 cells labeling CTSD at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1186255) at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).