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CTCF Antibody for ChIP, WB, IHC and IF
High-quality CTCF antibodies for chromatin organisation and epigenetics research.
CTCF antibodies are essential tools for studying CCCTC-binding factor (CTCF), a key regulator of chromatin architecture, transcriptional insulation, and genome organisation. As a critical protein in epigenetics and 3D genome structure, accurate detection of CTCF is essential for reproducible results in chromatin and gene regulation studies.
At Biorbyt, we offer a comprehensive range of CTCF antibodies validated for ChIP, WB, IHC, IF, IP and FC, including polyclonal, monoclonal and recombinant antibodies, with strong performance across human, mouse, and rat samples.
What is CTCF?
CTCF (CCCTC-binding factor) is a highly conserved zinc finger protein that acts as a key regulator of chromatin organisation and gene expression. It plays a central role in shaping the 3D genome by forming chromatin loops, defining topologically associating domains (TADs), and regulating enhancer-promoter interactions. Through its function as an insulator and transcriptional regulator, CTCF is essential for maintaining genomic stability and controlling epigenetic processes.
Recent research has expanded the understanding of CTCF beyond a structural protein to an active regulator of chromatin accessibility and gene expression. CTCF works closely with the cohesin complex to organise genome architecture, while also exhibiting cell-type-specific binding patterns that influence transcriptional programs. These findings highlight CTCF as a critical factor in epigenetics, development, and cancer biology, making it a widely studied target in ChIP, chromatin profiling, and genome-wide regulatory studies.
What are CTCF antibodies used for?
Key applications include:
- Chromatin immunoprecipitation (ChIP / ChIP-seq)
- Western blot (WB)
- Immunohistochemistry (IHC)
- Immunofluorescence (IF)
- Immunoprecipitation (IP)
- Flow cytometry (FC)
CTCF antibodies for ChIP: key requirements
CTCF is one of the most widely studied proteins in ChIP and ChIP-seq experiments, and antibody performance is critical for successful chromatin analysis.
A high-quality CTCF ChIP antibody should have:
- Strong enrichment at known CTCF binding sites
- High signal-to-noise ratio
- Minimal background binding
- Compatibility with crosslinked chromatin
Recommended ChIP antibodies:
These antibodies are validated for ChIP, IF, IHC, IP and WB, making them suitable for both ChIP-qPCR and ChIP-seq workflows.
KO validation and specificity in CTCF antibody selection
Knockout (KO) validation is one of the most reliable approaches for confirming antibody specificity, particularly in Western blot and imaging applications.
Why KO validation matters:
- Confirms loss of signal in CTCF knockout samples
- Reduces risk of non-specific binding
- Improves confidence in experimental results
When to prioritise KO-validated antibodies:
- Western blot (WB)
- ICC / IF
- Quantitative protein analysis
Recommended:
This antibody is validated in gene knockdown/knockout systems, ensuring high specificity for endogenous CTCF detection.
Recommended CTCF antibodies by application
SKU | Product Name | Applications | Size | Price |
|---|---|---|---|---|
orb1260507 | ChIP, IF, IHC, IP, WB | 100 μl | $830 | |
orb1261271 | ChIP, IF, IHC, IP, WB | 100 μl | $830 | |
orb340732 | ChIP, IF, IHC, WB | 30 μl | $130 | |
orb1951174 | FC, ICC, IHC, WB | 50 μl | $260 | |
orb1519896 | FC, ICC, IHC, IP, WB | 20 μl | $250 | |
orb2621233 | FC | 100 μg | $910 |
Mouse monoclonal vs rabbit antibodies for CTCF
Choosing between mouse monoclonal and rabbit-derived antibodies depends on the experimental design and required performance.
Mouse monoclonal antibodies
- Recognise a single epitope and offer high specificity
- May reduce background in certain assays
- Useful for co-staining experiments
Rabbit polyclonal antibodies
- Recognise multiple epitopes and provide higher sensitivity
- Often perform well in ChIP and WB applications
- Suitable for low-abundance targets
Recombinant rabbit monoclonal antibodies
- Combine specificity and reproducibility
- Reduce batch-to-batch variation
- Are ideal for long-term and quantitative studies
Recommended products:
Why choose Biorbyt CTCF antibodies?
- Validated for ChIP, WB, IHC, IF, IP and FC
- Strong performance in chromatin and epigenetics workflows
- Available in polyclonal, monoclonal and recombinant formats
- Suitable for human, mouse and rat samples
- Designed for genome organisation research
Conclusion
Biorbyt provides a comprehensive portfolio of CTCF antibodies for chromatin biology and epigenetics research, including ChIP-validated and KO-validated options, as well as monoclonal and recombinant formats.
By selecting antibodies based on validation strategy and application requirements, researchers can achieve reliable and reproducible results in genome organisation and gene regulation studies.