CTCF Antibody

• Catalog Number: orb340732
• Category: Antibodies
• Description: The CTCF Antibody is suitable for ChIP, IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human CTCF .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human CTCF
• UniProt ID: P49711, Q61164, Q9R1D1
• Tested applications: ChIP, IF, IHC, WB
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, ChIP: 1:20-1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Transcriptional repressor CTCF; 11-zinc finger pro
• Research Area: Cancer, Epigenetics
• Note: For research use only
• Entrez: 13018, 83726, 10664

Western blot analysis of CTCF expression in Hela (A), PC3 (B), COS7 (C) whole cell lysates. (Predicted band size: 45; 82 kD; Observed band size: 140 kD)

Immunohistochemical analysis of CTCF staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CTCF staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).