Anti-CTCF Antibody

• Catalog Number: orb340732
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CTCF
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IH, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: Recombinant full length protein of human CTCF
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, ChIP: 1:20-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CTCF
• Entrez: 83726, 10664, 13018
• UniProt ID: P49711, Q61164, Q9R1D1
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Transcriptional repressor CTCF; 11-zinc finger pro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CTCF expression in Hela (A), PC3 (B), COS7 (C) whole cell lysates. (Predicted band size: 45; 82 kD; Observed band size: 140 kD)

Immunohistochemical analysis of CTCF staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CTCF staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).