CRYAB/Alpha B Crystallin Antibody

• Catalog Number: orb1535136
• Category: Antibodies
• Description: CRYAB/Alpha B Crystallin Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6D11
• Tested applications: FC, IF, IHC, IHC-P, IP, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG1
• Immunogen: Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
• Concentration: 1 mg/ml
• Dilution range: Flo (1:100), IF (1:50 - 1:100), IHC, IHC-P (10 µg/ml), IP, WB (1:500 - 1:1000)
• Conjugation: Unconjugated
• Target: CRYAB / Alpha B Crystallin
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% rAlbumin
• Alternative names: CRYAB, Alpha-crystallin B chain, CRYA2, Crystallin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.

Western blot of extracts (35 ug) from 9 different cell lines by using anti-CRYAB monoclonal antibody.

Immunoprecipitation (IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.).

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.