Phospho-alpha B Crystallin (Ser59) Rabbit Polyclonal Antibody

SKU: orb155643

Description

Phospho-alpha B Crystallin (Ser59) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Hamster, Human, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human alpha B Crystallin around the phosphorylation site of Ser59 AP(p-S)W
• Target: CRYAB
• Molecular Weight: 20 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

alpha B Crystallin(phospho S59); alpha B Crystallin(phospho Ser59); p-alpha B Crystallin(S59); p-alpha B Crystallin(Ser59); AACRYA; alpha B crystallin; alpha crystallin B chain; alpha crystallin B chain; alpha(B) crystallin; alpha(B)-crystallin; alpha-crystallin B chain; CRYA2; CRYAB; CRYAB_HUMAN; Crystallin alpha B; Crystallin alpha polypeptide 2; CTPP 2; CTPP2; Heat shock 20 kD like protein; Heat shock protein beta 5; Heat shock protein beta-5; HSPB5; NY REN 27 antigen; Renal carcinoma antigen NY REN 27; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-alpha B Crystallin (Ser59)) Polyclonal Antibody, Unconjugated (orb155643) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-alpha B Crystallin (Ser59)) Polyclonal Antibody, Unconjugated (orb155643) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CRYAB

UniProt

P02511