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Catalog Number | orb1247644 |
---|---|
Category | Antibodies |
Description | CRYAB Antibody |
Target | CRYAB |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Reactivity | Mouse, Rat |
Predicted Reactivity | Human |
Form/Appearance | Liquid |
Concentration | 500 ug/mL |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Immunogen | The immunogen for this antibody is: C-RLEKDRFSVNLD |
UniProt ID | P02511 |
Tested applications | ELISA, WB |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 22kDa band observed in Mouse and Rat Eye lysates (calculated MW of 20.1kDa according to Mouse NP_034094.1 and Rat NP_037067.1). Recommended concentration: 0.03-0.1-ug/ml. |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | CRYAB, crystallin, alpha B, CRYA2, CTPP2, HSPB5, h Read more... |
Note | For research use only |
NCBI | NP_001876.1 |
orb1247644 (0.05 ug/ml) staining of Mouse (A) and Rat (B) Eye lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC, IHC-P, IP, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |