Crotonyl-HIST1H3A (K4) antibody

• Catalog Number: orb516380
• Category: Antibodies
• Description: Crotonyl-HIST1H3A (K4) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Peptide sequence around site of Crotonyl-Lys (4) derived from Human Histone H3.1
• Dilution range: WB:1:500-1:5000, IP:1:200-1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HIST1H3A
• UniProt ID: P68431
• Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Alternative names: H3 histone family member E pseudogene antibody; H3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Crotonyl-HIST1H3A (K4) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real

Western blot aanalysis of Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysateusing Crotonyl-HIST1H3A (K4) antibody

Immunoprecipitation analysis of 293 whole cell lysate using Crotonyl-HIST1H3A (K4) antibody