COX4I1 Antibody

SKU: orb1256931

Description

COX4I1 Antibody

Research Area

Cancer Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-169 of human COX IV (NP_001852.1).
• Target: COX4I1
• Molecular Weight: Observed: 17kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

COX4I1, COX4, COXIV, MGC72016, COX4-1

Western blot analysis of extracts of various cell lines, using COX IV Antibody (orb1256931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat spleen using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using COX IV antibody (orb1256931). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 22Rv1 cells, using 3 ug COX IV antibody (orb1256931). Western blot was performed from the immunoprecipitate using COX IV antibody (orb1256931) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

COX4I1

UniProt

P13073