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COX IV Antibody
SKU: orb1256931
Featured
Description
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 23-169 of human COX IV (NP_001852.1). |
| Target | COX4I1 |
| Molecular Weight | Observed: 17kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−COX4I1, COX4, COXIV, MGC72016, COX4-1
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Western blot analysis of extracts of various cell lines, using COX IV Antibody (orb1256931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat spleen using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using COX IV antibody (orb1256931). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of 22Rv1 cells, using 3 ug COX IV antibody (orb1256931). Western blot was performed from the immunoprecipitate using COX IV antibody (orb1256931) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
COX4I1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
COX IV Antibody (orb1256931)
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