CARD16 Antibody

SKU: orb1247658

Description

CARD16 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-ETLGLSAGPIPGN
• Target: CARD16
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CARD16, COP, CARD only protein, PSEUDO-ICE, COP1, caspase-1 dominant-negative inhibitor pseudo-ICE, pseudo interleukin-1beta converting enzyme, caspase recruitment domain family, member 16

orb1247658 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing mitochondrial staining. The nuclear stain is DAPI.

orb1247658 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

CARD16

UniProt

Q5EG05

RefSeq (Protein):NP_443121.1