Goat anti-COP1 / PSEUDO-ICE (Isofom 2) Antibody

• Catalog Number: orb18343
• Category: Antibodies
• Description: Goat polyclonal antibody to CARD16
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF
• Reactivity: Human
• Dilution range: ELISA: 1:4000, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 10.7
• Target: COP1 / PSEUDO-ICE (Isofom 2)
• Entrez: 114769
• Protein Sequence: ETLGLSAGPIPGN
• RRID: AB_10751642
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CARD16 antibody, anti COP antibody, anti CARD
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 28-30kDa band in human colon, pancreas, placenta and tonsil lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.7kDa according to NP_443121.1. The 28-30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using CARD16 antibody

Flow cytometric analysis of paraformaldehyde fixed A549 cells using CARD16 antibody