contactin 1 Antibody

• Catalog Number: orb1248300
• Category: Antibodies
• Description: contactin 1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-ENIRGKDKHQAR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 140kDa band observed in Human, Mouse, Rat and Pig Brain lysates (calculated MW of 113kDa according to NP_001834.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 140kDa
• Target: contactin 1
• UniProt ID: Q12860
• NCBI: NP_001242992.1, NP_001834.2, NP_778203.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CNTN1, contactin 1, F3, GP135, contactin-1, glycop
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 140kDa band observed in Human, Mouse, Rat and Pig Brain lysates (calculated MW of 113kDa according to NP_001834.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248300 (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248300 (0.3 ug/ml) staining of Pig Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248300 (5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.