contactin 1 Antibody

• Catalog Number: orb1248275
• Category: Antibodies
• Description: contactin 1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-QVTSQEYSARLEN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 140kDa band observed in Mouse Brain, and approx 150kDa observed in Human and Rat Brain lysates (calculated MW of 113kDa according to according to Human, NP_001834.2, Mouse NP_031753.1 and Rat NP_476459.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum) and Mouse Brain (Cerebral Cortex). Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 150kDa
• Target: contactin 1
• UniProt ID: Q12860
• NCBI: NP_001834.2, NP_778203.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CNTN1, contactin 1, F3, GP135, contactin-1, glycop
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 140kDa band observed in Mouse Brain, and approx 150kDa observed in Human and Rat Brain lysates (calculated MW of 113kDa according to according to Human, NP_001834.2, Mouse NP_031753.1 and Rat NP_476459.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum) and Mouse Brain (Cerebral Cortex). Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248275 (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248275 (5 ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, DAB-staining.

orb1248275 (1 ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248275 (5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.