contactin 1 Antibody

SKU: orb1248275

Description

contactin 1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 140kDa band observed in Mouse Brain, and approx 150kDa observed in Human and Rat Brain lysates (calculated MW of 113kDa according to according to Human, NP_001834.2, Mouse NP_031753.1 and Rat NP_476459.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum) and Mouse Brain (Cerebral Cortex). Recommended concentration: 5ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QVTSQEYSARLEN
• Target: contactin 1
• Molecular Weight: Approx 150kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

CNTN1, contactin 1, F3, GP135, contactin-1, glycoprotein gP135, neural cell surface protein F3

orb1248275 (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248275 (5 ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, DAB-staining.

orb1248275 (1 ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248275 (5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

contactin 1

UniProt

Q12860

RefSeq (Protein):NP_001834.2, NP_778203.1