COMT Antibody

SKU: orb1247659

Description

COMT Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: EIA, ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1). Recommended concentration: 0.03-0.1µg/mL.Immunohistochemistry:In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Recommended concentration: 2-4µg/mL. Paraffin embedded Human Testis and Small Intestine. Recommended concentration: 3.75µg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QDIIPQLKKKYDVD
• Target: COMT
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

COMT, catechol-O-methyltransferase

orb1247659 (0.3 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247659 (0.5 ug/ml) as the reporter antibody.

orb1247659 (4 ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1247659 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247659 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

COMT

UniProt

P21964

RefSeq (Protein):NP_009294.1, NP_000745.1