Goat anti-COMT (internal) Antibody

SKU: orb19316

Description

Goat polyclonal antibody to COMT

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.03-0.1 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IE: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2ug/ml.IHC: In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Recommended concentration, 2-4µg/ml.WB: Approx 28kDa band and faint 25kDa band observed in human testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: COMT (internal)
• Molecular Weight: 30.0; 24.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti COMT antibody, anti catechol-O-methyltransferase antibody

Immunohistochemical staining of human Cerebellum using COMT antibody

Western blot analysis of Human Testis lysate using COMT antibody

Immunohistochemical staining of Human Testis using COMT antibody

Immunohistochemical staining of Human Small Intestine using COMT antibody

Quick Database Links

Gene Symbol

COMT (internal)

NCBI Gene ID

1312