Collagen Type VI Antibody

• Catalog Number: orb345358
• Category: Antibodies
• Description: Collagen Type VI antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Bovine, Human
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.19 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Immunogen: Collagen Type VI from human and bovine placenta
• UniProt ID: P12109
• Tested applications: DOT, ELISA, IHC, IP, WB
• Dilution range: ELISA: 1:5,000 - 1:20,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:500 - 1:5,000
• Application notes: Anti-Collagen Type VI has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
• Antibody Type: Primary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: rabbit anti-Collagen Type VI antibody, Collagen al
• Note: For research use only
• NCBI: AAH52575.1

Dot Blot results using Rabbit Anti-Collagen VI Antibody. Sample: A) Human Collagen VI (p/n orb750386), B) Bovine/Human Collagen VI (p/n orb750380). Load: 1) 1000 ng, 2) 333.33 ng, 3) 111.11 ng, 4) 37.03 ng, 5) 12.35 ng. Primary Antibody: Rabbit Anti-Collagen VI antibody at 5 µg/ml at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated (p/n orb347673) 1:40000 at RT for 30 mins. Blocking: BlockOut® Universal Blocking Buffer (p/n orb348644) at RT for 30 mins.

Biorbyt anti collagen VI antibody (1:400 45 min RT) showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH6.0 at 99-100°C - 20 minutes for antigen retrieval.

Western blot results using Rabbit Anti-Collagen Type VI Antibody. Lane: 1) Human Collagen Type VI (p/n orb750386) reduced and boiled, 3) Human Collagen Type VI reduced and not boiled, 5) Opal prestain molecular weight marker, 7) Human Collagen Type VI not reduced and boiled, 9) Human Collagen Type VI not reduced and not boiled. Lanes 2, 4, 6, 8 blank. Load: 10 µg. Primary Antibody: Rabbit Anti-Collagen Type VI Antibody 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG HRP conjugated (p/n orb347673) 1:40000 at RT for 30 mins. Blocking: BlockOut® Universal Blocking Buffer (p/n orb348644) at RT for 1hr.