COL6A1-COL6A3 Antibody (Biotin)

SKU: orb345873

Description

Collagen Type VI antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:5,000 - 1:10,000
• Reactivity: Bovine, Human
• Application Notes:
  Anti-Collagen Type VI (RABBIT) Antibody Biotin Conjugated is suitable for western blot, immunoprecipitation, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Collagen Type VI from human and bovine placenta
• Target: COL6A1-COL6A3
• Purity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Collagen Type VI antibody biotin conjugation, biotin conjugated rabbit anti-Collagen Type VI antibody, Collagen alpha-1 (VI) chain, Collagen VI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody

Immunohistochemistry of Rabbit anti-collagen VI antibody. Tissue: human placenta (left), testis (right). Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH6.0 at 99-100°C - 20 minutes. Primary antibody: collagen VI antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: collagen VI is extracellular. Staining: collagen VI as precipitated red signal red staining of stromal and extracellular spaces in the placenta and extracellular spaces between seminiferous tubules in the testis, with hematoxylin purple nuclear counterstain.

Quick Database Links

Gene Symbol

COL6A1-COL6A3

UniProt

P12109

RefSeq:AAH52575.1