Collagen Type IV, human

• Catalog Number: orb27211
• Category: Antibodies
• Description: Mouse monoclonal antibody to Collagen IV
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: COL 94
• Tested applications: IHC-Fr, IHC-P, WB
• Isotype: IgG1
• Immunogen: Purified human Collagen IV
• Antibody Type: Primary Antibody
• Concentration: app. 100 µg/ml
• Dilution range: (liquid conc.) 1:10 - 1:50
• Conjugation: Unconjugated
• Hazard Information: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
• UniProt ID: P29400, P02462, P08572
• RRID: AB_10917193
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
• Note: For research use only
• Application notes: Positive Control: Human skin, placenta or lens capsule. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: 60 min at RT
• Expiration Date: 12 months from date of receipt.