Human Collagen Type IV Antibody

SKU: orb27211

Description

Mouse monoclonal antibody to Collagen IV

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IHC-Fr, IHC-P, WB
• Dilution Range: (liquid conc.) 1:10 - 1:50
• Application Notes:
  Positive Control: Human skin, placenta or lens capsule. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: 60 min at RT

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: COL 94
• Immunogen: Purified human Collagen IV
• Purification: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Concentration: app. 100 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P02462, P29400, P08572