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Collagen IV antibody
Description
Images & Validation
−| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:100-800, IHC-P: 1:100-500 (based on 0.5 mg/ml) |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Collagen IV. Please contact us for the exact immunogen sequence. The peptide is available as orb374961. |
| Target | Collagen IV |
| Molecular Weight | 161 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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WB analysis of rat bladder (lane 1), rat uterus (lane 2), rat testis (lane 3), mouse kidney (lane 4) using Collagen IV antibody (1 ug/ml)

Western blot analysis of rat brain (lane 1), U251 cells (lane 2), rat stomach (lane 3), mouse liver (lane 4) using Collagen IV antibody (3.5 ug/ml)

IHC-P staining of rat brain tissue using Collagen IV antibody (2.5 ug/ml)

IHC-P image of rat pancreas tissue using anti-Collagen IV (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat pancreas tissue using Collagen IV antibody (2.5 ug/ml)

IHC-P image of rat colon tissue using Collagen IV antibody (2.5 ug/ml)

Immunohistochemical staining of rat colon tissue using anti-Collagen IV (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse uterus tissue using anti-Collagen IV (2.5 ug/ml)
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Protocol Information
Collagen IV antibody (orb340147)
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