COL5A1 rabbit pAb

SKU: orb764900

Description

Rabbit polyclonal antibody to Collagen V

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Collagen V alpha1. AA range:301-350
• Molecular Weight: 200
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-COL5A1 antibody, anti-Collagen alpha-1(V) chain antibody

Western Blot analysis of various cells using COL5A1 Polyclonal Antibody.

Immunofluorescence analysis of HeLa cells, using Collagen V alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Collagen V alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa, and HUVEC, and HepG2 cells, using Collagen V alpha1 Antibody. The lane on the right is blocked with the synthesized peptide.