COL5A1 rabbit pAb

• Catalog Number: orb764900
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Collagen V
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Collagen V alpha1. AA range:301-350
• MW: 200
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-COL5A1 antibody, anti-Collagen alpha-1(V) cha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using COL5A1 Polyclonal Antibody.

Immunofluorescence analysis of HeLa cells, using Collagen V alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Collagen V alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa, and HUVEC, and HepG2 cells, using Collagen V alpha1 Antibody. The lane on the right is blocked with the synthesized peptide.