COL5A1 Antibody (N-term)

• Catalog Number: orb1938951
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB28077
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:1000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 183560 Da
• Target: This COL5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the N-terminal region of human COL5A1.
• UniProt ID: P20908
• NCBI: NP_000084.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Collagen alpha-1(V) chain, COL5A1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-COL5A1 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 184 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

COL5A1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35 ug/lane). This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).

COL5A1 Antibody (N-term) western blot analysis in U251 cell line lysates (35 ug/lane). This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).

Immunohistochemical analysis of paraffin-embedded Human heart tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis of paraffin-embedded Human uterus tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.