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| Catalog Number | orb767611 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Collagen IV |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen IV alpha2. AA range:151-200 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 150 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-COL4A2 antibody, anti-Collagen alpha-2(IV) ch Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of SH-SY5Y cells using COL4A2 Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of SH-SY5Y 293T NIH-3T3 COLO cells using COL4A2 Polyclonal Antibody diluted at 1:2000.
Immunofluorescence analysis of HeLa cells, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.
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