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Catalog Number | orb1938185 |
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Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB18892 |
Tested applications | FC, WB |
Reactivity | Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 167553 Da |
Target | This COL4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-186 amino acids from the N-terminal region of human COL4A2. |
UniProt ID | P08572 |
NCBI | NP_001837.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Collagen alpha-2(IV) chain, Canstatin, COL4A2 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-COL4A2 Antibody (N-term) at 1:1000 dilution + Mouse kidney whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 168 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
COL4A2 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.