COL4A1 Antibody (N-term)

SKU: orb37846

Description

Rabbit polyclonal antibody to Collagen IV

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:1000, IF/ICC: 1:25
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB19425
• Immunogen: Synthetic Peptide
• Target: COL4A1
• Molecular Weight: 160611
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-Arresten antibody, anti-COL4A1 antibody, anti-COL4A1 NC1 domain antibody, anti-COL4A2 antibody, anti-COL4A3 antibody, anti-COL4A4 antibody, anti-COL4A5 antibody, anti-collagen alpha-1(IV) chain antibody, anti-Collagen IV Alpha 1 Polypeptide antibody, anti-Collagen IV Alpha 2 Polypeptide antibody, anti-Collagen Of Basement Membrane Alpha 1 Chain antibody, anti-Collagen Of Basement Membrane Alpha 2 Chain antibody, anti-Collagen Type IV Alpha 1 antibody, anti-Collagen Type IV Alpha 2 antibody, anti-Collagen Type IV Alpha 3 antibody, anti-Collagen Type IV Alpha 4 antibody, anti-Collagen Type IV Alpha 5 antibody, anti-Collagen Type IV antibody

Western blot analysis of lysates from Hela cell line and human placenta tissue lysate (from left to right), using COL4A1 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Fluorescent image of U251 cells stained with COL4A1 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

All lanes: Anti-COL4A1 Antibody (N-term) at 1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: DU145 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HT-1080 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 161 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

COL4A1

UniProt

P02462

RefSeq (Protein):NP_001836.2

Lin, Sheng-Hsuan et al. KMUP-1 attenuates high glucose and transforming growth factor-β1-induced pro-fibrotic proteins in mesangial cells Mol Med Rep, 15, 4199-4206 (2017)

PubMed: 28440482