CMG1 Antibody

• Catalog Number: orb1247666
• Category: Antibodies
• Description: CMG1 Antibody
• Target: IFT74
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-KTIVDALHSTSGN
• UniProt ID: Q96LB3
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13;6:44.; PMID: 17166276). Recommended concentration: 0.2-0.6ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IFT74, CCDC2, CMG-1, FLJ22621, MGC111562, intrafla
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_079379.2, NP_001092692.1, AAK77221.1, NP_001092693.1

orb1247666 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247666 (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

A) orb1247666 (2.5 mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control.