IFT74 Rabbit Polyclonal Antibody

SKU: orb1819463

Description

The IFT74 Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, IHC, and WB applications. This antibody targets the Intraflagellar transport protein 74 homolog in Human, Monkey, Mouse, and Rat samples.

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
• Reactivity: Human, Monkey, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E.coli-derived human PELI3 recombinant protein (Position: M1-R375). Human PELI3 shares 90.1% amino acid (aa) sequence identity with mouse PELI3.
• Target: Intraflagellar transport protein 74 homolog
• Molecular Weight: 69 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IFT74; CCDC2; CMG1; Intraflagellar transport protein 74 homolog; Capillary morphogenesis gene 1 protein; CMG-1; Coiled-coil domain-containing protein 2

Quick Database Links

Gene Symbol

Intraflagellar transport protein 74 homolog

UniProt

Q96LB3