Cleaved PARP (Asp214) (H8) rabbit mAb Antibody

SKU: orb1946095

Description

Poly-ADP-ribose polymerase 1 (PARP-1), is a substrate of caspase-3 and caspase-7, both of which play a dominant role in apoptosis. PARP is cleaved into 89 and 24 kDa fragments at Asp214. The detection of these fragments is used as an indicator of caspase activation and apoptosis induction for many cell lines. Under normal conditions, PARP aids in the detection and repair of DNA damage. With 1-2 million copies per nucleus, PARP is also involved in poly (ADP-ribosyl)ation, a post-translational protein modification mechanism used to modify chromatin structure and regulate transcription. Decreased PARP activity has been shown to lead to loss of memory and neuronal cell death.

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: PARP-H8
• Immunogen: A synthetic peptide corresponding to residues surrounding Asp214 of human PARP
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1

Western blot analysis of HeLa cell extract untreated or treated with Staurosporine using Cleaved PARP (Asp214) antibody PARP-H8 at 0.001 ug/mL. Cat. #orb1946095.

Peptide blocking flow cytometric analysis of SK.N.MC cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with immunogen peptide (black) or treated and blocked with immunogen peptide (gold) or untreated and blocked with irrelevant peptide (dark blue) or treated and blocked with irrelevant peptide (purple) using PARP Cleaved Asp214 antibody PARP-H8 at 0.1 μg/mL. Cat. #orb1946095.

Flow cytometric analysis of SK.N.MC cells unstained untreated cells as negative control (blue) or stained untreated (red) or treated with staurosporine (green) using PARP Cleaved (Asp214) antibody PARP-H8 at 0.1 μg/mL. Cat. #orb1946095.

Western blot analysis of HeLa cell extract untreated or treated with staurosporine using 0.001 μg/mL Cleaved PARP (Asp214) antibody PARP-H8 Cat. #orb1946095 or Company C antibody at 0.05 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Flow cytometric analysis of SK.N.MC cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using PARP, Cleaved Asp214 antibody PARP-H8 (Cat. #orb1946095) or Company C antibody at 0.1 μg/mL (manufacturer's recommended concentration).

Quick Database Links

UniProt

P09874