Cleaved PARP (Asp214) (H8) rabbit mAb Antibody

• Catalog Number: orb1946095
• Category: Antibodies
• Description: Cleaved PARP (Asp214) (H8) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: PARP-H8
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgGk
• Immunogen: A synthetic peptide corresponding to residues surrounding Asp214 of human PARP
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P09874
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosy
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa cell extract untreated or treated with Staurosporine using Cleaved PARP (Asp214) antibody PARP-H8 at 0.001 ug/mL. Cat. #orb1946095.

Peptide blocking flow cytometric analysis of SK.N.MC cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with immunogen peptide (black) or treated and blocked with immunogen peptide (gold) or untreated and blocked with irrelevant peptide (dark blue) or treated and blocked with irrelevant peptide (purple) using PARP Cleaved Asp214 antibody PARP-H8 at 0.1 μg/mL. Cat. #orb1946095.

Flow cytometric analysis of SK.N.MC cells unstained untreated cells as negative control (blue) or stained untreated (red) or treated with staurosporine (green) using PARP Cleaved (Asp214) antibody PARP-H8 at 0.1 μg/mL. Cat. #orb1946095.

Western blot analysis of HeLa cell extract untreated or treated with staurosporine using 0.001 μg/mL Cleaved PARP (Asp214) antibody PARP-H8 Cat. #orb1946095 or Company C antibody at 0.05 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Flow cytometric analysis of SK.N.MC cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using PARP, Cleaved Asp214 antibody PARP-H8 (Cat. #orb1946095) or Company C antibody at 0.1 μg/mL (manufacturer's recommended concentration).