Cleaved PARP (Asp214) (H8) rabbit mAb PE conjugate Antibody

SKU: orb1946094

Description

Poly-ADP-ribose polymerase 1 (PARP-1), is a substrate of caspase-3 and caspase-7, both of which play a dominant role in apoptosis. PARP is cleaved into 89 and 24 kDa fragments at Asp214. The detection of these fragments is used as an indicator of caspase activation and apoptosis induction for many cell lines. Under normal conditions, PARP aids in the detection and repair of DNA damage. With 1-2 million copies per nucleus, PARP is also involved in poly (ADP-ribosyl)ation, a post-translational protein modification mechanism used to modify chromatin structure and regulate transcription. Decreased PARP activity has been shown to lead to loss of memory and neuronal cell death.

Images & Validation

• Tested Applications: FC
• Dilution Range: For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: PARP-H8
• Immunogen: A synthetic peptide corresponding to residues surrounding Asp214 of human PARP
• Purification: Protein A+G
• Conjugation: PE

Storage & Handling

• Storage: 2-8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.09% NaN3, 0.2% rAlbumin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1

Flow cytometric analysis of SK.N.MC cells unstained and untreated as negative control (blue) or stained untreated (red) or treated with staurosporine (green) using Cleaved PARP (Asp214) antibody PARP-H8 PE conjugate. Cat. #orb1946094.

Quick Database Links

UniProt

P09874