Cleaved LC3A Antibody

• Catalog Number: orb1787989
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-146 amino acids from human Cleaved LC3A.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: 110~146
• UniProt ID: Q9H492
• MW: 14272 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:25, WB: 1:1000, WB: 1:500, WB: 1:1000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Microtubule-associated proteins 1A/1B light chain
• Note: For research use only

Western blot analysis of lysates from A431, Hela cell line, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A) (upper) or Beta-actin (lower).

Western blot analysis of lysates from A431, Hela cell line, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A) (upper) or Beta-actin (lower).

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) Antibody (upper) or Beta-actin (lower).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse mouse embryonic fibroblasts cell line) cells labeling Pdx1 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). The nuclear counter stain is DAPI (blue). Immunofluorescence image showing cytoplasm on NIH/3T3 cell line.