Clatherin Light Chain Antibody (LCA/LCB)

• Catalog Number: orb386040
• Category: Antibodies
• Description: Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant N-terminal protein fragment from human Clathrin light chain was used as the immunogen for the Clathrin Light Chain antibody.
• UniProt ID: P09496, P09497
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Clathrin Heavy Chain antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CLC/1421
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Clathrin Light Chain antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human pancreas with Clathrin Light Chain antibody (clone CLC/1421). Required HIER: boil tissue sections in 10mM Citrate buffer, pH6.0, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Clathrin Light Chain Antibody (clone CLC/1421). Confirmation of Integrity and Purity of the Antibody.