Citrulline Antibody

• Catalog Number: orb396406
• Category: Antibodies
• Description: Mouse Anti-Citrullinated protein Monoclonal IgG1
• Target: Citrulline
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: All
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Protein G Purified
• Immunogen: Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH).
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC-P
• Application notes: A 1:1000 dilution of SMC-501 was sufficient for detection of Citrulline in 2 µg of Citrulline conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Clone Number: 6C2.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: L-Citrulline Antibody, 2-Amino-5-(carbamoylamino)p
• Note: For research use only

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Tissue: Colon tissue. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite.

Western Blot analysis of Citrulline-BSA Conjugate showing detection of 67 kDa Citrulline protein using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 μg). Lane 3: BSA (2.0 μg). Lane 4: Citrulline-BSA (0.5 μg). Lane 5: Citrulline-BSA (2.0 μg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.