ASS Antibody (Center)

• Catalog Number: orb1929777
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB20920
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Dilution range: IF: 1:25, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 46530 Da
• Target: This ASS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-221 amino acids from the Central region of human ASS.
• UniProt ID: P00966
• NCBI: NP_000041.2, NP_446464.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Argininosuccinate synthase, Citrulline--aspartate
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Staining ASS in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling ASS at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Western blot analysis of ASS Antibody (Center) in Hela cell line lysates (35 ug/lane). ASS (arrow) was detected using the purified Pab.

All lanes: Anti-ASS Antibody (Center) at 1:2000 dilution. Lane 1: MOLT-4 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lane 4: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ASS Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: MOLT-4 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ASS Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: MOLT-4 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ASS Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: PC-12 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: Mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.