Cisapride

SKU: orb1309100

Description

Cisapride

Research Area

Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 81098-60-4
• MW: 465.95
• Purity: 99.77%
• Formula: C₂₃H₂₉ClFN₃O₄
• SMILES: C(N[C@H]1[C@@H](OC)CN(CCCOC2=CC=C(F)C=C2)CC1)(=O)C3=C(OC)C=C(N)C(Cl)=C3
• Target: Potassium Channel,5-HT Receptor
• Solubility: DMSO:255 mg/mL (547.27 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.29 mM)

Bioactivity

• Target IC50:
  ERG channel (human):9.4 nM|5-HT4 receptor:140 nM(EC50)
• In Vitro:
  Cisapride inhibits vascular Kv current independently of serotonin 5-HT4-receptor activation. As HERG channel blocker, cisapride, can inhibit the growth of gastric cancer cells by altering distribution of cell cycle and inducing apoptosis so as to be of potential value in the treatment of gastric cancer. Cisapride could inhibit the growth and clonogenicity of human gastric cancer lines by specific blockage of HERG channel in a time- and dose-dependent manner while has little effects on GES cells.
• Cell Research:
  Cells in the logarithmic growth phase are harvested and seeded in 96-well plates. The cell number is diluted to 5000/well in 200 microliters of RPMI1640 medium. In vitro experiments are carried out on the gastric cancer cell lines and GES cell line, using an MTT proliferation assay. MTT stains live cells, and the optical density absorbance at 490 nM wavelength correlates with the cell number. Cisapride is added to the cells at concentrations of 0, 12.5, 25, 50, 100 or 200 nM and equivalent volume of ethanol is added as control in order to eliminate the effects of solvent. Twenty microliters of MTT is added to each well of the cell culture 4 h before termination of the culture at 37 C. One hundred and fifty microliters of dimethyl sulfoxide is added to each well at the end of the culture and the plate is agitated for 10 minutes. The absorbency at 490 nm is read by a BIOHIT BP800 plate reader. Growth curve is drawn according to MTT colorimetry. The inhibition rate is calculated.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cisaprid, Cisapride, 5HT Receptor, 5-HT Receptor, (±)-Cisaprid, 5-hydroxytryptamine Receptor, 5-HT4 agonist, 5-HT, 5HTReceptor, Inhibitor, gastrointestinal, hERG, patch-clamp, Kaudalit, inhibit, Kinestase, R 51619, Serotonin Receptor, PotassiumChannel, Potassium Channel, R-51619, R51619, Prepulsid, Pridesia, prokinetic agent

Compound Identifiers

PubChem CID

6917698