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Catalog Number | orb1246453 |
---|---|
Category | Antibodies |
Description | CHRNB2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: QPRHHCARQRLR |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 40-48 kDa band observed in Human Brain (Cerebellum),) lysates (calculated MW of 57.0 kDa according to NP_000739.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 2.5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CHRNB2 |
UniProt ID | P17787 |
NCBI | NP_000739.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | CHRNB2 , cholinergic receptor, nicotinic, beta 2 ( Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 40-48 kDa band observed in Human Brain (Cerebellum),) lysates (calculated MW of 57.0 kDa according to NP_000739.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 2.5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246453 (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1246453 (2.5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Bovine, Canine, Guinea pig, Human, Mouse, Porcine, Rat | |
Canine, Guinea pig, Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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