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| Catalog Number | orb1246453 |
|---|---|
| Category | Antibodies |
| Description | CHRNB2 Antibody |
| Target | CHRNB2 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rat |
| Form/Appearance | Liquid |
| Concentration | 500 ug/mL |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Immunogen | The immunogen for this antibody is: QPRHHCARQRLR |
| UniProt ID | P17787 |
| Tested applications | ELISA, IHC, WB |
| Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 40-48 kDa band observed in Human Brain (Cerebellum),) lysates (calculated MW of 57.0 kDa according to NP_000739.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 2.5ug/ml. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CHRNB2 , cholinergic receptor, nicotinic, beta 2 ( Read more... |
| Research Area | Cell Biology, Signal Transduction |
| Note | For research use only |
| NCBI | NP_000739.1 |

orb1246453 (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246453 (2.5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
IF, IHC-Fr, IHC-P, WB | |
Gallus, Human | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Human | |
0.16-10 ng/mL | |
0.056 ng/mL |
WB | |
Bovine, Canine, Porcine, Zebrafish | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Porcine, Zebrafish | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Mouse, Porcine, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
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