CHP2 Antibody / Calcineurin B homologous protein 2

SKU: orb2643774

Description

CHP2 (calcineurin B homologous protein 2), also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.

Images & Validation

• Tested Applications: IF, WB
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CHP2 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2c, kappa
• Clone No.: CPTC-CHP2-1
• Immunogen: Recombinant human full-length protein was used as the immunogen for the CHP2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of permeabilized human MCF7 cells with CHP2 antibody (green) and Reddot nuclear stain (red).

Western blot testing of human MCF7 and PC3 cell lysate with CHP2 antibody. Predicted molecular weight ~22 kDa.

SDS-PAGE analysis of purified, BSA-free CHP2 antibody as confirmation of integrity and purity.

Quick Database Links

UniProt

O43745