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Item 1 of 3
CHP2 Antibody / Calcineurin B homologous protein 2
Catalog Number: orb639919
| Catalog Number | orb639919 |
|---|---|
| Category | Antibodies |
| Description | CHP2 (calcineurin B homologous protein 2), also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway. |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | Mouse IgG2c, kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Immunogen | Recombinant human full-length protein was used as the immunogen for the CHP2 antibody. |
| Tested applications | IF, WB |
| Dilution range | Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml |
| Application notes | Optimal dilution of the antibody should be determined by the researcher. |
| Antibody Type | Primary Antibody |
| Clone Number | CPTC-CHP2-1 |
| Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Hazard Information | This CHP2 antibody is available for research use only. |
| Note | For research use only |
Immunofluorescent staining of permeabilized human MCF7 cells with CHP2 antibody (green) and Reddot nuclear stain (red).
Western blot testing of human MCF7 and PC3 cell lysate with CHP2 antibody. Predicted molecular weight ~22 kDa.
SDS-PAGE analysis of purified, BSA-free CHP2 antibody as confirmation of integrity and purity.
