Choline O-acetyltransferase (ChAT) Antibody

SKU: orb3167536

Description

Our Anti-Choline O-acetyltransferase (ChAT) rabbit polyclonal primary antibody detects guinea pig, mouse, pig, rabbit, and rat Choline O-acetyltransferase (ChAT), and is whole serum. It is validated for use in ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded.

Images & Validation

• Tested Applications: ELISA, ICC, IHC
• Dilution Range: IHC: 1:400-1:2000; ICC: 1:400-1:2000
• Reactivity: Guinea pig, Mouse, Porcine, Rabbit, Rat
• Application Notes:
  IHC/ICC on 4% PFA or formalin fixed frozen sections; paraffin sections can be more difficult and require more extensive antigen recovery methods. 1:400-1:2000 depending up detection and incubation times ELISA: direct antigen ELISA This antiserum will superbly stain both cell bodies and nerve terminal, and works particularly well in enteric and peripheral neurons.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Mixed
• Immunogen: A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH
• Target: Choline O-acetyltransferase (ChAT)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Choline O-acetyltransferase; CHOACTase; Choline acetylase

Quick Database Links

Gene Symbol

Choline O-acetyltransferase (ChAT)

UniProt

P13222