ChAT (Choline Acetyltransferase) Antibody

SKU: orb1401953

Description

Our Anti-ChAT (Choline Acetyltransferase) primary antibody from Biorbyt is goat polyclonal. It detects chicken, guinea pig, human, mouse, and non-human primate, and rat ChAT (Choline Acetyltransferase) and is affinity purified.

Research Area

Disease Biomarkers, Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB: 0.111111111, IHC: 1:100-1:1000
• Reactivity: Gallus, Guinea pig, Human, Mouse, Primate, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human choline acetyltransferase
• Target: ChAT (Choline Acetyltransferase)
• Molecular Weight: 70
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 5 mg/ml rAlbumin + 0.2% NaN3
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Acetyl CoA choline O acetyltransferase antibody, Acetyl CoA:choline O acetyltransferase antibody, ChAT antibody, CHOACTase antibody, Choline acetylase antibody, choline acetyltransferase antibody, Choline O acetyltransferase antibody, Choline O-acetyltransferase antibody, CLAT_HUMAN antibody, CMS1A antibody, CMS1A2 antibody, EC 2.3.1.6 antibody, OTTHUMP00000019583 antibody, OTTHUMP00000019584 antibody

Immunohistochemistry detection of ChAT in Coronal section of rat neocortex tissue using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. orb1401953). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. orb1401953) was incubated with the sample. orb1401953 showing the choline acetyltransferase positive cholinergic nerve fibers and terminals.

Immunohistochemistry detection of ChAT in rat organotypic slice cultures of septum tissue using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. orb1401953). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. orb1401953) was incubated with the sample at 1:100.

Immunostaining of rat brainstem showing specific labeling using our anti-ChAT antibody.

Western blot detection of ChAT using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. orb1401953). Gel was loaded with A431 (Lane1), C2C12 lysate (Lane2), HEK293 lysate (Lane 3), Hela lysate (Lane 4), HepG2 lysate (Lane 5), Human Placenta lysate (Lane 6), Huvec lysate (Lane 7), Jurkat lysate (Lane 8), L6 lysate (Lane 9), Mouse brain lysate (Lane 10), NIH/3T3 lysate (Lane 11), PC12 lysate (Lane 12), PC3 lysate (Lane 13), RAW264.7 lysate(Lane 14. Proteins were transferred to the PVDF membrane, blocked, and then washed with a buffer. Primary antibody was incubated with sample at 1:1000 and then detected via chemiluminescence using rabbit anti-goat secondary antibody conjugated to HRP. Arrow indicates protein ChAT (~68-70 kDa).

Western blot detection of ChAT using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. orb1401953). Gel was loaded with Mouse brain lysate. Proteins were transferred to the PVDF membrane, blocked, and then washed with a buffer. Primary antibody was incubated with sample at 1:1000 and then detected via chemiluminescence using rabbit anti-goat secondary antibody conjugated to HRP. Arrow indicates protein ChAT (~68-70 kDa).

Western blot detection of ChAT using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. orb1401953). Gel was loaded with NIH/3T3 lysate. Proteins were transferred to the PVDF membrane, blocked, and then washed with a buffer. Primary antibody was incubated with sample at 1:1000 and then detected via ECL using rabbit anti-goat secondary antibody conjugated to HRP. Arrow indicates protein ChAT (~68-70 kDa).

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~70 kDa ChAT.

Quick Database Links

Gene Symbol

ChAT (Choline Acetyltransferase)

UniProt

P28329