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CHK2 Antibody
Description
Images & Validation
−| Tested Applications | ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; IF/ICC: 1/200 - 1/1000; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b |
| Clone No. | 1C12B8 |
| Immunogen | Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli. |
| Target | Disease |
| Molecular Weight | 61kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Confocal immunofluorescence analysis of Hela cells using CHK2 antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using CHK2 antibody

Western blot analysis of cell lysate from various cell types using CHK2 antibody
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Protocol Information
CHK2 Antibody (orb97589)
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