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Description
Images & Validation
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| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Gallus |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | Chicken IgG F(ab')2 fragment |
| Purity | This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Chicken IgG, Chicken IgG F(ab’)2 and Chicken Serum. No reaction was observed against Chicken IgG F(c). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 90 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Chicken IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated [orb346918]
DOT, ELISA, IHC, WB
Gallus
Rabbit
Polyclonal
AP
1 mgChicken IgG F(ab')2 Fluorescein Antibody [orb346057]
SDS-PAGE
FITC
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken IgG, anti-Chicken IgG F(ab’)2 and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(c) or anti-Pepsin.
Chicken
1 mgChicken IgG F(ab')2 Peroxidase Antibody [orb346061]
SDS-PAGE
HRP
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken IgG, anti-Chicken IgG F(ab’)2 and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(c) or anti-Pepsin.
Chicken
1 mgChicken IgG F(ab')2 Biotin Antibody [orb346066]
SDS-PAGE
Biotin
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Chicken IgG, anti-Chicken IgG F(ab’)2 and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(c) or anti-Pepsin.
Chicken
1 mgChicken IgG F(ab')2 Agarose Antibody [orb346049]
SDS-PAGE
Agarose
This product is an purified Chicken IgG isolated from normal Chicken Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5 mg of anti-Chicken IgG.
Chicken
5 mg

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