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Description
Research Area
Images & Validation
−| Tested Applications | SDS-PAGE |
|---|---|
| Application Notes |
Key Properties
−| Source | Chicken |
|---|---|
| Biological Origin | Chicken |
| Isotype | IgG F(ab')2 |
| Conjugation | Agarose |
| Purity | This product is an purified Chicken IgG isolated from normal Chicken Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5 mg of anti-Chicken IgG. |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. DO NOT FREEZE. |
|---|---|
| Form/Appearance | Suspension of agarose beads |
| Buffer/Preservatives | Preservative: 0.5% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Reduced Chicken IgG Fab Fragment (p/n orb2652771). Lane 4: Reduced Chicken IgM Whole Molecule (p/n orb346054). Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.
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Protocol Information
Chicken IgG F(ab')2 Agarose Antibody (orb346049)
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