CHEK2 Antibody

• Catalog Number: orb1261835
• Category: Antibodies
• Description: CHEK2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human CHEK2 (NP_009125.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 60kDa
• Target: CHEK2
• UniProt ID: O96017
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Chk2 Antibody: CDS1, CHK2, LFS2, RAD53, hCds1, HuC
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CHEK2 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human lung cancer using CHEK2 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using CHEK2 antibody. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A549 cells using CHEK2 antibody.

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug CHEK2 antibody. Western blot was performed from the immunoprecipitate using CHEK2 antibody (orb1261835) at a dilition of 1:1000.