CHAT Antibody

• Catalog Number: orb1260518
• Category: Antibodies
• Description: CHAT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 331-630 of human CHAT (NP_001136401.1).
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 70kDa
• Target: CHAT
• UniProt ID: P28329
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ChAT, CMS1A, CMS1A2, choline acetyltransferase, ac
• Note: For research use only
• Application notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CHAT antibody (orb1260518) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of NIH/3T3 cells using CHAT antibody (orb1260518) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of THP-1 cells using CHAT antibody (orb1260518) at dilution of 1:100. Blue: DAPI for nuclear staining.