CHAF1B Antibody

• Catalog Number: orb1257128
• Category: Antibodies
• Description: CHAF1B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-559 of human CHAF1B (NP_005432.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 70kDa
• Target: CHAF1B
• UniProt ID: Q13112
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: CHAF1B, CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPH
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CHAF1B antibody (orb1257128) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat fallopian tube using CHAF1B antibody (orb1257128) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using CHAF1B antibody (orb1257128) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using CHAF1B antibody (orb1257128) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF-7 cells using CHAF1B antibody (orb1257128). Blue: DAPI for nuclear staining.