CFTR Antibody / Cystic Fibrosis Transmembrane Regulator

• Catalog Number: orb750073
• Category: Antibodies
• Description: Cystic fibrosis transmembrane conductance regulator (CFTR) is involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CFTR/1785
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A partial recombinant protein corresponding to amino acids 258-385 from the human protein was used as the immunogen for this CFTR antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CFTR antibody is available for research use only.
• Entrez: 1080
• UniProt ID: P13569
• Storage: Store the CFTR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CFTR antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas with CFTR antibody (clone CFTR/1785). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free CFTR Antibody (clone CFTR/1785). Confirmation of Integrity and Purity of the Antibody.