CEP290 Antibody

SKU: orb1249429

Description

CEP290 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 80kDa band in Human Kidney, Testis, and HeLa lysates after 1µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 290kDa according to NP_079390.3. The 80kDa band was successfully blocked by incubation with the immunizing peptide.Immunocytochemistry: Formaldehyde-fixed cell lines derived from Prostate Cancer and from Breast Cancer show typical centrosomal staining. Recommended concentration: 2-3µg/mL

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RNSKHLKQQQYRAEN
• Target: CEP290
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CEP290, centrosomal protein 290kDa, 3H11Ag, BBS14, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, NPHP6, SLSN6, rd16, CTCL tumor antigen se2-2, monoclonal antibody 3H11 antigen, nephrocytsin-6, prostate cancer antigen T21

Quick Database Links

Gene Symbol

CEP290

UniProt

O15078

RefSeq (Protein):NP_079390.3