Goat anti-CEP290 Antibody

SKU: orb20381

Description

Goat polyclonal to CEP290

Images & Validation

• Tested Applications: ELISA, ICC, IHC
• Dilution range: ELISA: 1:128000, IHC-P: 5-7 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 80kDa band in Human Kidney, Testis, and HeLa lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 290kDa according to NP_079390.3. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CEP290
• Molecular Weight: 290
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CEP290 antibody, anti centrosomal protein 290kDa antibody, anti 3H11Ag, BBS14, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, NPHP6, SLSN6, rd16 antibody, anti CTCL tumor antigen se2-2 antibody, anti monoclonal antibody 3H11 antigen antibody, anti nephrocytsin-6 antibody, anti prostate cancer antigen T21 antibody

Immunofluorescence analysis of cell lines OPCT-1 (Lane 1) and MDA468 (Lane 2) using CEP290 antibody

Quick Database Links

Gene Symbol

CEP290

NCBI Gene ID

80184