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Catalog Number | orb20381 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to CEP290 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IHC |
Reactivity | Human |
Dilution range | ELISA: 1:128000, IHC-P: 5-7 μg/ml |
Conjugation | Unconjugated |
MW | 290 |
Target | CEP290 |
Entrez | 80184 |
Protein Sequence | RNSKHLKQQQYRAEN |
RRID | AB_10747948 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti CEP290 antibody, anti centrosomal protein 290 Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 80kDa band in Human Kidney, Testis, and HeLa lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 290kDa according to NP_079390.3. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of cell lines OPCT-1 (Lane 1) and MDA468 (Lane 2) using CEP290 antibody